Effect of pore size on the performance of immobilised enzymes.
نویسندگان
چکیده
Porous materials are widely employed as supports in the immobilisation of enzymes. Traditionally macroporous materials with pore diameters >50 nm were believed to be the most suitable support material, ensuring no spatial restrictions upon enzyme molecules entering such large pores. In recent years however, there has been growing emphasis in the use of mesoporous supports with pore diameters ranging between 2 and 50 nm. It is thought this smaller pore range may offer enhanced conformational stability to immobilised enzymes while not being so small as to restrict enzyme access. Despite their increasing popularity, many argue that mesoporous materials have not yet proven superior to traditional macroporous supports for enzyme immobilisation. Through the design and application of a unique confidence rating system we were able to accurately compare data and establish trends between pore characteristics and protein loading. By analysing published data (182 experiments in total) and extracting pore characteristics and protein loading values, we have described three categories of pore diameters in which correlations between pore characteristics and protein loading are noted. With pore diameters less than 10 nm we see a general decrease in protein loading as the enzymes find physical restrictions in accessing the high surface offered in this pore diameter range. At pore sizes greater than 100 nm, protein loading generally decreases due to a concomitant reduction in available surface area. In the pore range of 10-100 nm there it is expected to see a decrease in protein loading level with increasing pore diameter. In fact protein loading in this range remains largely constant, suggesting some degree of protein-protein interaction blocking pores and restricting access to the increasing surface area available at decreasing pore diameters. No trends were established between pore characteristics and retention of activity.
منابع مشابه
Studying Performance of Dubinin-astakhov and Dubinin-raduchkevic Equations to Evaluate Nanopore Volume and Pore Size of MCM-41 Particles (TECHNICAL NOTE)
MCM-41 particles were synthesized using inorganic raw materials and Cetyltrimethylammonium bromide (CTAB). The textural properties and structure of MCM-41 particles were characterized by XRD, SEM, TEM and N2 adsorption-desorption methods. To study performance of Dubinin-Astakhov and Dubinin-Raduchkevic isotherm models in evaluating mesopore volume and pore size of MCM-41 samples, the mesopore ...
متن کاملInvestigating the Influence of Filter Uniformity Coefficient and Effective Pore Size on Critical Hydraulic Gradient and Maximum Erosion of Dispersive and Non-dispersive Samples
Filter is one of the main components of embankment dams. By a simple but effective performance, filter protects the dam against erosion and soil scouring in impervious core caused by leakage (piping) and makes it safe. Interaction between filter and erodible base soil is a complex phenomenon which is dependent upon several factors, and has challenged researchers for better understanding the fil...
متن کاملPreparation of AlPO4-5 nano-pore crystals: Effect of alteration in precursor gel preparation on the morphology of crystals
Aluminophosphate-5 (AlPO4-5) nano-pore zeolite was synthesized. The effect of alteration in precursor gel preparation on the morphology of zeolite was studied. The effect of variety of synthesis conditions on the purity, crystal and pore size, and crystallinity of AlPO4-5 zeolite been studied. A hydrothermal synthesis has been used to produce nano-pore Aluminophosphate-5 c...
متن کاملPreparation of AlPO4-5 nano-pore crystals: Effect of alteration in precursor gel preparation on the morphology of crystals
Aluminophosphate-5 (AlPO4-5) nano-pore zeolite was synthesized. The effect of alteration in precursor gel preparation on the morphology of zeolite was studied. The effect of variety of synthesis conditions on the purity, crystal and pore size, and crystallinity of AlPO4-5 zeolite been studied. A hydrothermal synthesis has been used to produce nano-pore Aluminophosphate-5 c...
متن کاملSensitivity Analysis of the Effect of Pore Structure and Geometry on Petrophysical and Electrical Properties of Tight Media: Random Network Modeling
Several methodologies published in the literature can be used to construct realistic pore networks for simple rocks, whereas in complex pore geometry formations, as formed in tight reservoirs, such a construction still remains a challenge. A basic understanding of pore structure and topology is essential to overcome the challenges associated with the pore scale modeling of tight porous media. A...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical Society reviews
دوره 42 23 شماره
صفحات -
تاریخ انتشار 2013